MSB® Spin PCRapace

Item No.: 1020220300 & 1020220400

Highlights:

Timesaving: pure PCR products in 7 minutes
Fewer centrifugation steps: 2-step bind-and-elute protocol
Flexible use: clean-up of PCR and enzymatic reactions, suitable for the up concentration of samples, using a minimal elution volume of 10 μl
High recovery: 95% recovery for fragment sizes of
80 bp – 30 kb

Product Details

With a processing time of about 7 minutes, the MSB^® Spin PCRapace is one of the fastest systems for clean-up of PCR products from dNTPs, primers, enzymes, dyes, additives, and salts. The kit is also a powerful and efficient tool for the up concentration of PCR products. The clean-up procedure requires only two simple steps: binding of the DNA to a membrane, which is achieved at low, nucleic acid-sparing, salt concentrations, and the elution of the DNA fragments immediately after binding.

Specifications
Downstream application	Sequencing, cloning, ligation, restriction enzymes digestion, PCR
Target nucleic acid	DNA
Starting material	up to 100 µl reaction volume: PCR reaction mixture restriction digestion mixture ligation mixture cDNA synthesis mixture cycle sequencing reaction mixture
Recovery	80 – 95 %, depends on fragment length
Size range	Fragment sizes of 80 bp – 30 kb can be purified
Preparation time	approx. 7 min
Elution Volume	10 - 50 µl
Technology	Spin column
Certification	RUO

Resources

Ordering Details

Product Name	Package Size	Catalogue No.
MSB^® Spin PCRapace	250 purifications	1020220300
MSB^® Spin PCRapace	500 purifications	1020220400

FAQs

Can the MSB® Spin PCRapace be used for sample clean up prior 16S rRNA sequencing?

Yes, the MSB® Spin PCRapace is perfectly suitable for removing impurities originating from PCR reactions, like primers, enzymes, and short DNA fragments. With the MSB® Spin PCRapace fragments of 80 bp-30 kb are recovered.