The InviScreen® Fish DNA Detection Kit provides a real-time PCR method for the qualitative detection of fish DNA in food, feed, and surface swabs. The assay is based on the amplification of species-specific DNA using hydrolysis probes, which can be performed with a variety of food matrices. The kit allows simultaneous amplification of the allergenic target species and an internal amplification control (IAC) to exclude false negative results due to PCR inhibition. The real-time PCR method can be performed in commonly available PCR instruments with FAM and VIC/HEX fluorescence channels. Under optimal conditions, the kit provides highly sensitive detection of at least 10 mg allergenic substance / kg food sample.
Specifications | |
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Sample preparation | For DNA extraction the use of InviSorb® Spin Food Kit is recommended |
LOD (Detection Limit) | 10 ppm |
Specificity | Cartilaginous (Chondrichthyes) and bony fish (Osteichthyes) |
No. of tests | 100 |
Technology | qPCR / Real-Time PCR |
Instrument compatibility | Applied Biosystems™ 7500, Agilent AriaMx™, Bio Rad CFX96 Touch™ and CFX96 Touch™ Deep Well |
Dyes | Channel Target: FAM, Channel IAC: HEX |
Certification | RUO |
Product Name | Package Size | Catalogue No. |
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InviScreen® Fish DNA Detection Kit | 100 reactions | 6012014200 |